POIR.01.01.01-00-0406/18 An automatic device for preparing samples of a dry matrix droplet
1 753 709,75 zł

The aim of the project is to design and manufacture an automatic die die cutter equipped with a system that fully controls the cutting process.
The project assumes that the resulting device will collect soaked tissue paper from the warehouse, analyze the parameters of a drop dried on the paper and position and cut a disc of a defined diameter to a multi-well plate.
This will allow you to achieve several basic goals: determining the correctness of sample collection, the possibility of cutting several discs from one soaked field, re-cutting – in case it is necessary to cut the previously cut tissue paper again, or planning to cut as many discs as possible from one sheet. An important functionality is to determine the droplet size. On this basis, a correction factor will be determined that takes into account the relationship between the droplet size and the concentration of the substances to be determined. This part of the project will be combined with the measurements of dry matrix samples.
Additional functionalities of the cutter being created will be the possibility of preparing samples for the determination of elements, mainly iron. This requires the development of punches and dies made of non-ferrous materials to avoid contamination of the sample, but at the same time to maintain high cut quality. The device will also enable the preparation of samples for DNA analysis. To make this possible, it is necessary to use a system that removes the dust generated during cutting, as well as cleaning the knife and matrix
after each cut.
The purpose of the research and development work carried out is to create a device that will minimize human work and the related errors in sample preparation.